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Western blot analysis of PHAP III expression in human A549 (A) and HepG2 (B) cells, and rat testis (C) with PHAP antibody III at 1 μg/mL.
Immunohistochemistry of PHAP III in human small intestine tissue with PHAP III antibody at 2 μg/mL.
Immunofluorescence of PHAP III in Human Small Intestine cells with PHAP III antibody at 10 μg/mL.
Western blot analysis of PHAP III expression in human A549 (A) and HepG2 (B) cells, and rat testis (C) with PHAP antibody III at 1 μg/mL.
Trial Size

PHAP III Antibody

CATALOG NUMBER: 3147

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PHAP III antibody was raised with a synthetic peptide corresponding to amino acids close to carboxy terminus of human PHAP III.
The immunogen is located within amino acids 200 - 250 of PHAP III.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
PHAP III antibody can be used for detection of PHAP III by Western blot at 1 μg/mL. A band at approximately 35 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human and rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
PHAP III has no cross-reaction to PHAP I and PHAP I2a.
Positive Control 1:
Cat. No. 1203 - A549 Cell Lysate
Positive Control 2:
Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 3:
Cat. No. 1308 - Human Small Intestine Tissue Lysate
Positive Control 4:
Cat. No. 11-801 - Human Small Intestine Tissue Slide
Predicted Molecular Weight:
35 kDa
Purification:
PHAP III Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PHAP III Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PHAP III antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures..
Ncbi Official Symbol:
ANP32E
Additional Names:
PHAP III Antibody: LANPL, LANP-L, Acidic leucine-rich nuclear phosphoprotein 32 family member E, LANP-like protein
Protein Accession Number:
NP_112182
Protein Gi Number:
13569879
Ncbi Gene Id Number:
81611
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PHAP III Antibody: Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.
Background Reference 1:
Jiang X, Kim HE, Shu H, Zhao Y, Zhang H, Kofron J, Donnelly J, Burns D, Ng SC , Rosenberg S, Wang X. Distinctive roles of PHAP proteins and prothymosin-α in a death regulatory pathway. Science. 2003;299(5604):223-6.
Background Reference 2:
Nicholson DW, Thornberry NA. Apoptosis. Life and death decisions. Science. 2003 10;299(5604):214-5.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3147

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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