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Antibody used in IHC on Human Stomach.
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Antibody used in IHC on Human Stomach.

PHB Antibody

CATALOG NUMBER: 27-716

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PHB.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
PHB antibody can be used for detection of PHB by ELISA at 1:1562500. PHB antibody can be used for detection of PHB by western blot at 1.0-2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
30 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store PHB antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
PHB
Additional Names:
PHB, PHB1, HEL-215, HEL-S-54e
Protein Accession Number:
NP_002625
Protein Gi Number:
4505773
Ncbi Gene Id Number:
5245
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Prohibitin is from an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.
Background Reference 1:
Fraering, P.C., et al., (2004) Biochemistry 43 (30), 9774-9789.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-716

List Size:
100 ul

List Price:

$519.00

Datasheet
Shipping Info

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