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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PHB antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of PHB Antibody in Hela cell line lysates (35ug/lane)
Western blot analysis of PHB using rabbit polyclonal PHB Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PHB gene.
Confocal immunofluorescent analysis of PHB Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PHB antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

PHB Antibody

CATALOG NUMBER: 61-438

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken
Immunogen:
This PHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the Central region of human PHB.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
30 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PHB
Additional Names:
Prohibitin, PHB
Protein Accession Number:
P35232
Protein Gi Number:
464371
Ncbi Gene Id Number:
5245
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Prohibitin is an evolutionarily conserved protein that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region.
Background Reference 1:
Gregory-Bass,R.C., Int. J. Cancer 122 (9), 1923-1930 (2008)
Background Reference 2:
Ross,J.A., J. Biol. Chem. 283 (8), 4699-4713 (2008)
Background Reference 3:
White,J.J., Genomics 11 (1), 228-230 (1991)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-438

List Size:
400 ul

List Price:

$495.00

Datasheet
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