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Western blot analysis of extracts of various cell lines, using PHB antibody (13-904).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse stomach using PHB antibody (13-904) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using PHB antibody (13-904) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using PHB antibody (13-904) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using PHB antibody (13-904) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using PHB antibody (13-904) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using PHB antibody (13-904) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using PHB antibody (13-904).
Immunofluorescence analysis of HepG2 cells using PHB antibody (13-904).
Western blot analysis of extracts of various cell lines, using PHB antibody (13-904).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

PHB Antibody [A9D4]

CATALOG NUMBER: 13-904

Clonality:
Monoclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of human PHB
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:50
Positive Control 1:
293T
Positive Control 2:
Jurkat
Positive Control 3:
Mouse kidney
Positive Control 4:
HepG2
Predicted Molecular Weight:
Observed: 30kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PHB
Additional Names:
PHB, PHB1, HEL-215, HEL-S-54e
Ncbi Gene Id Number:
5245
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-904

List Size:
50 uL

List Price:

$335.00

Out of stock

Datasheet
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