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Antibody used in IHC on Human Stomach.
Antibody used in WB on Human 293T at 1.25 ug/ml.
Antibody used in IHC on Human Stomach.

PHF1 Antibody

CATALOG NUMBER: 27-772

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PHF1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
PHF1 antibody can be used for detection of PHF1 by ELISA at 1:1562500. PHF1 antibody can be used for detection of PHF1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
293T Cell Lysate
Predicted Molecular Weight:
62 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store PHF1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
PHF1
Additional Names:
PHF1, PCL1, PHF2, hPHF1, MTF2L2, TDRD19C
Protein Accession Number:
NP_077084
Protein Gi Number:
13435397
Ncbi Gene Id Number:
5252
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PHF1 has significant sequence similarity with Drosophila Polycomblike. It contains a zinc finger-like PHD (plant homeodomain) finger which is distinct from other classes of zinc finger motifs and which shows the typical Cys4-His-Cys3 arrangement. PHD finger genes are thought to belong to a diverse group of transcriptional regulators possibly affecting eukaryotic gene expression by influencing chromatin structure. This gene encodes a protein with significant sequence similarity to Drosophila Polycomblike. The encoded protein contains a zinc finger-like PHD (plant homeodomain) finger which is distinct from other classes of zinc finger motifs and which shows the typical Cys4-His-Cys3 arrangement. PHD finger genes are thought to belong to a diverse group of transcriptional regulators possibly affecting eukaryotic gene expression by influencing chromatin structure. Two transcript variants have been found for this gene.
Background Reference 1:
Yang, X.J., (2001) J. Biol. Chem. 276 (46), 43065-43073.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-772

List Size:
100 ul

List Price:

$473.00

Datasheet
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