Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of PHF14 in 293 cell lysate with PHF14 antibody at 1 μg/ml.
Immunofluorescence of PHF14 in 293 cells with PHF14 antibody at 5 μg/mL.
Western blot analysis of PHF14 in 293 cell lysate with PHF14 antibody at 1 μg/ml.
Trial Size

PHF14 Antibody

CATALOG NUMBER: 7485

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PHF14 antibody was raised against a 19 amino acid peptide near the amino terminus of human PHF14.
The immunogen is located within amino acids 210 - 260 of PHF14 .
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
PHF14 antibody can be used for detection of PHF14 by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
PHF14 antibody is human, mouse and rat reactive. Multiple isoforms of PHF14 are known to exist.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2:
Cat. No. 17-010 - HEK293 Cell Slide
Predicted Molecular Weight:
Predicted: 98 kDa
Observed: 100kDa
Purification:
PHF14 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PHF14 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PHF14 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
PHF14
Additional Names:
PHF14 Antibody : KIAA0783, PHD finger protein 14
Protein Accession Number:
NP_055475
Protein Gi Number:
55769548
Ncbi Gene Id Number:
9678
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PHF14, a novel plant homology domain (PHD) transcription factor, controls the proliferation of mesenchymal cells by regulating PDGFR alpha expression in embryonic development (1). PHD finger transcription factors are concerned with transcriptional regulation by interacting with modified histones (2), and dysregulation of several genes encoding PHD finger transcription factors, such as ING1 and RBP2, has been found in human cancer diseases suggesting these factors have an important role for cancer cell proliferation. PHF14 is a potential target for new treatments of lung fibrosis (1, 3).
Background Reference 1:
Kitagawa M, Takebe A, Ono Y, et al. Phf14, a novel regulator of mesenchyme growth via platelet-derived growth factor (PDGF) receptor-a. J. Biol. Chem. 2012; 287:27983-96.
Background Reference 2:
Musco G and Peterson P. PHD finger of autoimmune regulator: an epigenetic link between the histone modifications and tissue-specific antigen expression in thymus. Epigenetics 2008; 3:310-4.
Background Reference 3:
Musselman CA and Kutateladze TG. PHD fingers: epigenetic effectors and potential drug targets. Mol. Interv. 2009; 9:314-23.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7485

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369