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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.

PHF5A Antibody

CATALOG NUMBER: 57-082

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This PHF5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-110 amino acids from the C-terminal region of human PHF5A.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
12 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PHF5A
Additional Names:
PHD finger-like domain-containing protein 5A, PHD finger-like domain protein 5A, Splicing factor 3B-associated 14 kDa protein, SF3b14b, PHF5A
Protein Accession Number:
Q7RTV0
Protein Gi Number:
46577625
Ncbi Gene Id Number:
84844
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a subunit of the splicing factor 3bprotein complex. Splicing factor 3b, together with splicing factor3a and a 12S RNA unit, forms the U2 small nuclearribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3acomplex binds pre-mRNA upstream of the intron's branch site in asequence-independent manner and may anchor the U2 snRNP to thepre-mRNA. The protein encoded by this gene contains aPHD-finger-like domain that is flanked by highly basic N- andC-termini. This protein belongs to the PHD-finger superfamily andmay act as a chromatin-associated protein. This gene has severalpseudogenes on different chromosomes.
Background Reference 1:
Kuwasako, K., et al. Proteins 71(4):1617-1636(2008)
Background Reference 2:
Will, C.L., et al. RNA 10(6):929-941(2004)
Background Reference 3:
Collins, J.E., et al. Genome Biol.(10), R84 (2004) :
Background Reference 4:
Will, C.L., et al. EMBO J. 21(18):4978-4988(2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-082

List Size:
400 ul

List Price:

$495.00

Datasheet
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