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Western blot analysis of PHGDH Antibody in mouse brain tissue lysates (35ug/lane)
Western blot analysis of PHGDH using rabbit polyclonal PHGDH Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHGDH gene (Lane 2).
Formalin-fixed and paraffin-embedded human brain tissue reacted with PHGDH Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of hela cells using PHGDH Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of PHGDH Antibody in mouse brain tissue lysates (35ug/lane)

PHGDH Antibody

CATALOG NUMBER: 61-612

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Pig
Immunogen:
This PHGDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-168 amino acids from the N-terminal region of human PHGDH.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
57 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PHGDH
Additional Names:
D-3-phosphoglycerate dehydrogenase, 3-PGDH, PHGDH, PGDH3
Protein Accession Number:
O43175
Ncbi Gene Id Number:
26227
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
3-Phosphoglycerate dehydrogenase catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.
Background Reference 1:
Klomp,L.W., et.al., Am. J. Hum. Genet. 67 (6), 1389-1399 (2000)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-612

List Size:
400 ul

List Price:

$495.00

Datasheet
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