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PHGDH Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Ramos cell line lysates (15ug/lane).
PHGDH Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

PHGDH Antibody

CATALOG NUMBER: 61-613

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Monkey
Immunogen:
This PHGDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 249-277 amino acids from the Central region of human PHGDH.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
57 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PHGDH
Additional Names:
D-3-phosphoglycerate dehydrogenase, 3-PGDH, PHGDH, PGDH3
Protein Accession Number:
O43175
Ncbi Gene Id Number:
26227
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95)catalyzes the transition of 3-phosphoglycerate into3-phosphohydroxypyruvate, which is the first and rate-limiting stepin the phosphorylated pathway of serine biosynthesis, usingNAD+/NADH as a cofactor.
Background Reference 1:
Du, H., et al. Reproduction 139(1):237-245(2010)
Background Reference 2:
Burton, R.L., et al. Biochemistry 48(22):4808-4815(2009)
Background Reference 3:
Kim, J.W., et al. Psychiatr. Genet. 19 (3), 161 (2009) :
Background Reference 4:
Tabatabaie, L., et al. Hum. Mutat. 30(5):749-756(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-613

List Size:
400 ul

List Price:

$495.00

Datasheet
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