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46-178 (3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-178 (3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

PHLDA2 Antibody

CATALOG NUMBER: 46-178

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-EPSRPSPQPKPRTP
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave no signal but low background in Jurkat and human placenta extracts at up to 1ug/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Placenta shows staining of the syncytiotrophoblasts. Recommended concentration, 3-6ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PHLDA2
Additional Names:
PHLDA2, pleckstrin homology-like domain, family A, member 2, TSSC3, IPL, BRW1C, BWR1C, HLDA2, PHLDA2, tumor suppressing subtransferable candidate 3, tumor-supressing STF cDNA 3, imprinted in placenta and liver, p17-Beckwith-Wiedemann region 1C, pleckstrin homology-like domain family A member 2, tumor suppressing subchromosomal transferable fragment cDNA 3, tumor suppressing subtransferable candidate 3, tumor-supressing STF cDNA 3
Protein Accession Number:
NP_003302.1
Protein Gi Number:
4507705
Ncbi Gene Id Number:
7262
Background Reference 1:
Qian N, Frank D, O'Keefe D, Dao D, Zhao L, Yuan L, Wang Q, Keating M, Walsh C, Tycko B. The IPL gene on chromosome 11p15.5 is imprinted in humans and mice and is similar to TDAG51, implicated in Fas expression and apoptosis. Hum Mol Genet. 1997 Nov;6(12):2021-9.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-178

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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