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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
PHLDA2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).

PHLDA2 Antibody

CATALOG NUMBER: 57-773

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PHLDA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-119 amino acids from the C-terminal region of human PHLDA2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
17 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PHLDA2
Additional Names:
Pleckstrin homology-like domain family A member 2, Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene C protein, Imprinted in placenta and liver protein, Tumor-suppressing STF cDNA 3 protein, Tumor-suppressing subchromosomal transferable fragment candidate gene 3 protein, p17-Beckwith-Wiedemann region 1 C, p17-BWR1C, PHLDA2, BWR1C, HLDA2, IPL, TSSC3
Protein Accession Number:
Q53GA4
Protein Gi Number:
84029394
Ncbi Gene Id Number:
7262
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is located in a cluster of imprinted genes onchromosome 11p15.5, which is considered to be an important tumorsuppressor gene region. Alterations in this region may beassociated with the Beckwith-Wiedemann syndrome, Wilms tumor,rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, andbreast cancer. This gene has been shown to be imprinted, withpreferential expression from the maternal allele in placenta andliver.
Background Reference 1:
O'Seaghdha, C.M., et al. Hum. Mol. Genet. 19(21):4296-4303(2010)
Background Reference 2:
Edenberg, H.J., et al. Alcohol. Clin. Exp. Res. 34(5):840-852(2010)
Background Reference 3:
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Background Reference 4:
Tang, K.F., et al. Biochim. Biophys. Acta 1770(5):820-825(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-773

List Size:
400 ul

List Price:

$495.00

Datasheet
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