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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PHLDA3 with C-terminal MYC tag probed with 46-179 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 46-179 (1mg/ml) in Lane B. Primary
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PHLDA3 with C-terminal MYC tag probed with 46-179 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 46-179 (1mg/ml) in Lane B. Primary

PHLDA3 Antibody

CATALOG NUMBER: 46-179

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-RARQSLGTGTLVS
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:In transfected HEK293 transiently expressing full-length Human PHLDA3 (myc and DYKDDDDK tagged), a band of approx. 18kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration: 0.2-1ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PHLDA3
Additional Names:
PHLDA3, TIH1, pleckstrin homology-like domain, family A, member 3, pleckstrin homology-like domain, family A, member 2, OTTHUMP00000033920
Protein Accession Number:
NP_036528.1
Protein Gi Number:
6912590
Ncbi Gene Id Number:
23612
Background Reference 1:
Frank D, Mendelsohn CL, Ciccone E, Svensson K, Ohlsson R, Tycko B. A novel pleckstrin homology-related gene family defined by Ipl/Tssc3, TDAG51, and Tih1: tissue-specific expression, chromosomal location, and parental imprinting. Mamm Genome. 1999 Dec;10(12):1150-9.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-179

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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