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Western blot analysis of various lysates using Phospho-CDC37-S13 Rabbit mAb at 1:1000 dilution. Both 293T cells and NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% BSA. | Detection: ECL Basic Kit. | Exposure time: 10s.
Western blot analysis of lysates from C6 cells, using Phospho-CDC37-S13 Rabbit mAb at 1:1000 dilution. C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% BSA. | Detection: ECL Basic Kit. | Exposure time: 3min.
Western blot analysis of various lysates using Phospho-CDC37-S13 Rabbit mAb at 1:1000 dilution. Both 293T cells and NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% BSA. | Detection: ECL Basic Kit. | Exposure time: 10s.

Phospho-CDC37-S13 Rabbit Recombinant Antibody (84-618)

CATALOG NUMBER: 84-618

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:500 - 1:2000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
293T,NIH/3T3,C6
Predicted Molecular Weight:
44kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CDC37
Additional Names:
P50CDC37; Phospho-CDC37-S13
Protein Accession Number:
Q16543
Ncbi Gene Id Number:
11140
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-618

List Size:
100 uL

List Price:

$540.00

Datasheet
Shipping Info

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