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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Cdc6-S54 Rabbit mAb at 1:1000 dilution. NIH/3T3 cells were treated with Paclitaxel (1 uM) at 37℃ for 20 hours. NIH/3T3 cells were treated with Hydroxyurea (4 mM) at 37℃ for 20 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% BSA. | Detection: ECL Basic Kit. | Exposure time: 5s.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Cdc6-S54 Rabbit mAb at 1:1000 dilution. NIH/3T3 cells were treated with Paclitaxel (1 uM) at 37℃ for 20 hours. NIH/3T3 cells were treated with Hydroxyurea (4 mM) at 37℃ for 20 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% BSA. | Detection: ECL Basic Kit. | Exposure time: 5s.

Phospho-Cdc6-S54 Rabbit Recombinant Antibody (84-626)

CATALOG NUMBER: 84-626

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:1000 - 1:6000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
NIH/3T3 treated with Paclitaxel,NIH/3T3 treated with Hydroxyurea
Predicted Molecular Weight:
63kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CDC6
Additional Names:
CDC18L; HsCDC6; MGORS5; HsCDC18; Phospho-Cdc6-S54
Protein Accession Number:
Q99741
Ncbi Gene Id Number:
990
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-626

List Size:
100 uL

List Price:

$540.00

Datasheet
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