Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of lysates from NIH/3T3 cells using Phospho-CDK2-Y15 Rabbit mAb at 1:1000 dilution. NIH/3T3 cells were treated with Hydroxyurea (4 Mm) at 37℃ for 20 hours or treated with Nocodazole (100 ng/mL) at 37℃ for 24 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% BSA. | Detection: ECL Basic Kit. | Exposure time: 3 s.
Western blot analysis of lysates from HeLa cells using Phospho-CDK2-Y15 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. HeLa cells were treated with Hydroxyurea (4 Mm) at 37℃ for 20 hours at 37℃ for 24 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3 % nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 3 s.
Western blot analysis of lysates from NIH/3T3 cells using Phospho-CDK2-Y15 Rabbit mAb at 1:1000 dilution. NIH/3T3 cells were treated with Hydroxyurea (4 Mm) at 37℃ for 20 hours or treated with Nocodazole (100 ng/mL) at 37℃ for 24 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% BSA. | Detection: ECL Basic Kit. | Exposure time: 3 s.

Phospho-CDK2-Y15 Rabbit Recombinant Antibody (84-581)

CATALOG NUMBER: 84-581

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:1000 - 1:10000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
NIH/3T3 treated with Hydroxyurea, NIH/3T3 treated with Nocodazole,HeLa treated with Hydroxyurea
Predicted Molecular Weight:
34 kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CDK2
Additional Names:
CDKN2; p33(CDK2); Phospho-CDK2-Y15
Protein Accession Number:
P24941
Ncbi Gene Id Number:
1017
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-581

List Size:
100 uL

List Price:

$540.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369