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Western blot analysis of lysates from HUVEC cells using Phospho-eNOS-S1177 Rabbit mAb at 1:6000 dilution incubated overnight at 4℃. HUVEC cells were treated with Pervanadate (100 μM) at 37℃ for 10 minutes. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 60 s.
Western blot analysis of lysates from HUVEC cells using Phospho-eNOS-S1177 Rabbit mAb at 1:6000 dilution incubated overnight at 4℃. HUVEC cells were treated with Pervanadate (100 μM) at 37℃ for 10 minutes. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 60 s.

Phospho-eNOS-S1177 Rabbit Recombinant Antibody (84-689)

CATALOG NUMBER: 84-689

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:2000 - 1:8000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
133 kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NOS3
Additional Names:
eNOS; ECNOS; Phospho-eNOS-S1177
Protein Accession Number:
P29474
Ncbi Gene Id Number:
4846
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-689

List Size:
100 uL

List Price:

$540.00

Datasheet
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