Western blot analysis of lysates from Jurkat cells, using Phospho-FOXO1-T24 + FOXO3A-T32 + FOXO4-T28 Rabbit mAb at 1:2000 dilution. Jurkat cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 20s.

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-FOXO1-T24 + FOXO3A-T32 + FOXO4-T28 Rabbit mAb at 1:2000 dilution. NIH/3T3 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 20s.

Western blot analysis of lysates from Jurkat cells, using Phospho-FOXO1-T24 + FOXO3A-T32 + FOXO4-T28 Rabbit mAb at 1:2000 dilution. Jurkat cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 20s.

Phospho-FOXO1-T24 + FOXO3A-T32 + FOXO4-T28 Rabbit Recombinant Antibody (84-677)

CATALOG NUMBER: 84-677

Clonality:

Monoclonal

Tested Applications:

ELISA, WB

Host Species:

Rabbit

Species Reactivity:

Human, Mouse

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human, Mouse

Conjugate:

Unconjugated

Tested Applications:

ELISA, WB

Application Note:

WB,1:1000 - 1:5000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Positive Control 1:

Jurkat treated with Calyculin A,NIH/3T3 treated with Calyculin A

Predicted Molecular Weight:

70kDa/71kDa/48kDa/54kDa

Advanced Validation

Properties

purification:

Affinity purification

Clonality:

Monoclonal

Isotype:

IgG

physical-state:

Liquid

Buffer:

Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.

Concentration:

Batch dependent

Storage Conditions:

Store at -20℃. Avoid freeze / thaw cycles.

Additional Info

Ncbi Official Symbol:

FOXO1/FOXO3A/FOXO4

Additional Names:

FoxO1/FoxO3a/FoxO4; Phospho-FOXO1-T24 + FOXO3A-T32 + FOXO4-T28

Protein Accession Number:

Q12778/O43524/P98177

Ncbi Gene Id Number:

2308/2309/4303

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

This product is for research use only.

CATALOG NUMBER:

84-677

List Size:

100 uL

List Price:

$540.00

Datasheet

Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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Phospho-FOXO1-T24 + FOXO3A-T32 + FOXO4-T28 Rabbit Recombinant Antibody (84-677)

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Conjugate:

Tested Applications:

Application Note:

Positive Control 1:

Predicted Molecular Weight:

purification:

Clonality:

Isotype:

physical-state:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Protein Accession Number:

Ncbi Gene Id Number:

User Note:

Background:

Disclaimer:

List Size:

Shipping Info

Customize your order

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