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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-GSK3α-S21+GSK3β-S9 Rabbit mAb at 1:800 dilution. NIH/3T3 cells were treated with 10% FBS at 37℃ for 30 minutes after serum-starvation overnight. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30s.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-GSK3α-S21+GSK3β-S9 Rabbit mAb at 1:800 dilution. NIH/3T3 cells were treated with 10% FBS at 37℃ for 30 minutes after serum-starvation overnight. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30s.

Phospho-GSK3α-S21+GSK3β-S9 Rabbit Recombinant Antibody (84-653)

CATALOG NUMBER: 84-653

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
NIH/3T3 treated with FBS
Predicted Molecular Weight:
51kDa/46kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
GSK3A/GSK3B
Additional Names:
GSK3B; GSK 3 beta; GSK3A; GSK 3 alpha; Phospho-GSK3α-S21+GSK3β-S9
Protein Accession Number:
P49840/P49841
Ncbi Gene Id Number:
2931/2932
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-653

List Size:
100 uL

List Price:

$540.00

Datasheet
Shipping Info

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