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Western blot analysis of lysates from C2C12 cells using Phospho-HDAC4-S246 + HDAC5-S259 + HDAC9-S220 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours. C2C12 treated with CIP (1 U/ul) at 37℃ for 1 hour. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30 s.
Western blot analysis of lysates from HeLa cells using Phospho-HDAC4-S246 + HDAC5-S259 + HDAC9-S220 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours. HeLa treated with λpp (2 U/ul) at 37℃ for 1 hour. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 60 s.
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Phospho-HDAC4-S246 + HDAC5-S259 + HDAC9-S220 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using Phospho-HDAC4-S246 + HDAC5-S259 + HDAC9-S220 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Phospho-HDAC4-S246 + HDAC5-S259 + HDAC9-S220 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Western blot analysis of lysates from C2C12 cells using Phospho-HDAC4-S246 + HDAC5-S259 + HDAC9-S220 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours. C2C12 treated with CIP (1 U/ul) at 37℃ for 1 hour. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30 s.

Phospho-HDAC4-S246 + HDAC5-S259 + HDAC9-S220 Rabbit Recombinant Antibody (84-687)

CATALOG NUMBER: 84-687

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
WB,1:500 - 1:1000, IHC-P,1:100 - 1:400, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
293T treated by EGF
Predicted Molecular Weight:
57-65kDa/97-122kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HDAC4/HDAC5/HDAC9
Additional Names:
HDAC4/HDAC5/HDAC9; Phospho-HDAC4-S246 + HDAC5-S259 + HDAC9-S220
Protein Accession Number:
P56524/Q9UQL6/Q9UKV0
Ncbi Gene Id Number:
9759/10014/9734
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-687

List Size:
100 uL

List Price:

$540.00

Datasheet
Shipping Info

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