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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Histone H3.3-T3 Rabbit mAb at 1:1000 dilution. NIH/3T3 cells were treated with Nocodazole (50 ng/mL) at 37℃ for 20 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% BSA. | Detection: ECL Enhanced Kit. | Exposure time: 3min.
Western blot analysis of lysates from HeLa cells, using Phospho-Histone H3.3-T3 Rabbit mAb at 1:1000 dilution. HeLa cells were treated with nocodazole (50 ng/mL) at 37℃ for 20 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% BSA. | Detection: ECL Basic Kit. | Exposure time: 1s.
Western blot analysis of lysates from C6 cells, using Phospho-Histone H3.3-T3 Rabbit mAb at 1:1000 dilution. C6 cells were treated with Nocodazole (50 ng/mL) at 37℃ for 20 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% BSA. | Detection: ECL Enhanced Kit. | Exposure time: 90s.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Histone H3.3-T3 Rabbit mAb at 1:1000 dilution. NIH/3T3 cells were treated with Nocodazole (50 ng/mL) at 37℃ for 20 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% BSA. | Detection: ECL Enhanced Kit. | Exposure time: 3min.

Phospho-Histone H3.3-T3 Rabbit Recombinant Antibody (84-625)

CATALOG NUMBER: 84-625

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Other (Wide Range Predicted), Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Other (Wide Range Predicted), Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
HeLa treated with Nocodazole,NIH/3T3 treated with Nocodazole,C6 treated with Nocodazole
Predicted Molecular Weight:
15kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
H3-3A
Additional Names:
H3F3; H3-3B; H3.3A; H3F3A; BRYLIB1; Phospho-Histone H3.3-T3
Protein Accession Number:
Q16695/P68431
Ncbi Gene Id Number:
8290/8350
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-625

List Size:
100 uL

List Price:

$540.00

Datasheet
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