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Western blot analysis of lysates from 3T3-L1 cells, using Phospho-HSL-S660 Rabbit mAb at 1:1000 dilution. 3T3-L1 cells that were not differentiated or differentiated into adipocytes treated with 0. 06mol/L alcohol and treated with isoproterenol (10 μM, 20 min). | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 180s.
Western blot analysis of lysates from 3T3-L1 cells, using Phospho-HSL-S660 Rabbit mAb at 1:1000 dilution. 3T3-L1 cells that were not differentiated or differentiated into adipocytes treated with 0. 06mol/L alcohol and treated with isoproterenol (10 μM, 20 min). | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 180s.

Phospho-HSL-S660 Rabbit Recombinant Antibody (84-708)

CATALOG NUMBER: 84-708

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
3T3-L1 treated with isoproterenol
Predicted Molecular Weight:
117kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
LIPE
Additional Names:
HSL; LHS; REH; AOMS4; FPLD6; Phospho-HSL-S660
Protein Accession Number:
Q05469
Ncbi Gene Id Number:
3991
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-708

List Size:
100 uL

List Price:

$540.00

Datasheet
Shipping Info

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