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Western blot analysis of lysates from Karpas 299 cells using Phospho-IRAK4-Ser346 Rabbit mAb at 1:1000 dilution. Karpas 299 cells were treated with IL-1β (200 ng/ml) at 37℃ for 15 minutes after serum starvation overnight. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 75s.
Western blot analysis of lysates from Karpas 299 cells using Phospho-IRAK4-Ser346 Rabbit mAb at 1:1000 dilution. Karpas 299 cells were treated with IL-1β (200 ng/ml) at 37℃ for 15 minutes after serum starvation overnight. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 75s.

Phospho-IRAK4-Ser346 Rabbit Recombinant Antibody (84-722)

CATALOG NUMBER: 84-722

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
Karpas 299 treated with IL-1β
Predicted Molecular Weight:
52kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
IRAK4
Additional Names:
IPD1; IMD67; REN64; IRAK-4; NY-REN-64
Protein Accession Number:
Q9NWZ3
Ncbi Gene Id Number:
51135
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-722

List Size:
100 uL

List Price:

$540.00

Datasheet
Shipping Info

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