Western blot analysis of lysates from HeLa cells, using Phospho-KAP1/TRIM28-S824 Rabbit mAb at 1:5000 dilution. HeLa cells were treated with Etoposide (25 uM) at 37℃ for 5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 60s.

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-KAP1/TRIM28-S824 Rabbit mAb at 1:5000 dilution. NIH/3T3 cells were treated with Etoposide (25 uM) at 37℃ for 5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 60s.

Western blot analysis of lysates from HeLa cells, using Phospho-KAP1/TRIM28-S824 Rabbit mAb at 1:5000 dilution. HeLa cells were treated with Etoposide (25 uM) at 37℃ for 5 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Enhanced Kit. | Exposure time: 60s.

Phospho-KAP1/TRIM28-S824 Rabbit Recombinant Antibody (84-686)

CATALOG NUMBER: 84-686

Clonality:

Monoclonal

Tested Applications:

ELISA, WB

Host Species:

Rabbit

Species Reactivity:

Human, Mouse

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human, Mouse

Conjugate:

Unconjugated

Tested Applications:

ELISA, WB

Application Note:

WB,1:1000 - 1:5000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Predicted Molecular Weight:

79kDa/89kDa

Advanced Validation

Properties

purification:

Affinity purification

Clonality:

Monoclonal

Isotype:

IgG

physical-state:

Liquid

Buffer:

Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.

Concentration:

Batch dependent

Storage Conditions:

Store at -20℃. Avoid freeze / thaw cycles.

Additional Info

Ncbi Official Symbol:

TRIM28

Additional Names:

KAP1; TF1B; RNF96; TIF1B; PPP1R157; TIF1beta; Phospho-KAP1/TRIM28-S824

Protein Accession Number:

Q13263

Ncbi Gene Id Number:

10155

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

This product is for research use only.

CATALOG NUMBER:

84-686

List Size:

100 uL

List Price:

$540.00

Datasheet

Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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Phospho-KAP1/TRIM28-S824 Rabbit Recombinant Antibody (84-686)

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Conjugate:

Tested Applications:

Application Note:

Predicted Molecular Weight:

purification:

Clonality:

Isotype:

physical-state:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Protein Accession Number:

Ncbi Gene Id Number:

User Note:

Background:

Disclaimer:

List Size:

Shipping Info

Customize your order

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