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Western blot analysis of lysates from HeLa cells using Phospho-NF-kB p65/RelA-S468 Rabbit mAb at 1:2000 dilution. HeLa cells were treated with TNF-α (20 ng/ml) and Calyculin A (100 nM) at 37℃ for 10 minutes. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30s.
Western blot analysis of lysates from NIH/3T3 cells using Phospho-NF-kB p65/RelA-S468 Rabbit mAb at 1:16000 dilution incubated overnight at 4℃. NIH/3T3 cells were treated with TNF-α (50 ng/mL) at 37℃ for 30 minutes | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3 % nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 20s.
Western blot analysis of lysates from C6 cells using Phospho-NF-kB p65/RelA-S468 Rabbit mAb at 1:16000 dilution incubated overnight at 4℃. C6 cells were treated with TNF-α (20 ng/ml) and Calyculin A (50 nM) at 37℃ for 30 minutes | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3 % nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 20s.
Immunoprecipitation of Phospho-NF-kB p65/RelA-S468 in 200 µg extracts from HeLa cells treated with TNF-α and Calyculin A using 0.5 µg Phospho-NF-kB p65/RelA-S468 Rabbit mAb. Western blot analysis was performed using Phospho-NF-kB p65/RelA-S48 Rabbit mAb at 1:3000 dilution.
Western blot analysis of lysates from HeLa cells using Phospho-NF-kB p65/RelA-S468 Rabbit mAb at 1:2000 dilution. HeLa cells were treated with TNF-α (20 ng/ml) and Calyculin A (100 nM) at 37℃ for 10 minutes. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30s.

Phospho-NF-kB p65/RelA-S468 Rabbit Recombinant Antibody (84-723)

CATALOG NUMBER: 84-723

Clonality:
Monoclonal
Tested Applications:
ELISA, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IP, WB
Application Note:
WB,1:2000 - 1:20000, IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
60kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
RELA
Additional Names:
p65; CMCU; NFKB3; AIF3BL3; Phospho-NF-kB p65/RelA-S468
Protein Accession Number:
Q04206
Ncbi Gene Id Number:
5970
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-723

List Size:
100 uL

List Price:

$540.00

Datasheet
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