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Western blot analysis of lysates from NIH/3T3 cells using Phospho-p38 MAPK-Y182 Rabbit mAb at 1:9000 dilution incubated overnight at 4℃. NIH/3T3 cells were treated with UV at room temperature for 15-30 minutes. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30s.
Western blot analysis of lysates from C6 cells using Phospho-p38 MAPK-Y182 Rabbit mAb at 1:9000 dilution incubated overnight at 4℃. C6 cells were treated with anisomycin (25 μg/mL) at 37℃ for 30 minutes after. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Mouse colon (untreated) and Mouse colon (lambda phosphatase-treated) tissue using Phospho-p38 MAPK-Y182 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat testis (untreated) and Rat testis (lambda phosphatase-treated) tissue using Phospho-p38 MAPK-Y182 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of HeLa cells (untreated) and HeLa cells (treated with λpp) using Phospho-p38 MAPK-Y182 Rabbit mAb followed by a further incubation with Cy3-conjugated Goat Anti-Rabbit IgG (H+L) (Red). The cells were counterstained with α-Tubulin Mouse mAb followed by incubation with PSFlo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of NIH/3T3 cells (untreated) and NIH/3T3 cells (treated with λpp) using Phospho-p38 MAPK-Y182 Rabbit mAb followed by a further incubation with Cy3-conjugated Goat Anti-Rabbit IgG (H+L) (Red). The cells were counterstained with α-Tubulin Mouse mAb followed by incubation with PSFlo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of PC-12 cells (untreated) and PC-12 cells (treated with λpp) using Phospho-p38 MAPK-Y182 Rabbit mAb followed by a further incubation with Cy3-conjugated Goat Anti-Rabbit IgG (H+L) (Red). The cells were counterstained with α-Tubulin Mouse mAb followed by incubation with PSFlo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Western blot analysis of lysates from NIH/3T3 cells using Phospho-p38 MAPK-Y182 Rabbit mAb at 1:9000 dilution incubated overnight at 4℃. NIH/3T3 cells were treated with UV at room temperature for 15-30 minutes. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30s.

Phospho-p38 MAPK-Y182 Rabbit Recombinant Antibody (84-670)

CATALOG NUMBER: 84-670

Clonality:
Monoclonal
Tested Applications:
ELISA, IF/ICC, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF/ICC, IHC-P, WB
Application Note:
WB,1:5000 - 1:10000, IHC-P,1:200 - 1:800, IF/ICC,1:50 - 1:200, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
41kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MAPK14
Additional Names:
RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA; Phospho-p38 MAPK-Y182
Protein Accession Number:
Q16539
Ncbi Gene Id Number:
1432
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-670

List Size:
100 uL

List Price:

$540.00

Datasheet
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