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Western blot analysis of various lysates using Phospho-PAK4/PAK5/PAK6-S474/S560/S602 Rabbit mAb at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated with PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% BSA. | Detection: ECL Basic Kit. | Exposure time: 10s.
Western blot analysis of various lysates using Phospho-PAK4/PAK5/PAK6-S474/S560/S602 Rabbit mAb at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated with PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% BSA. | Detection: ECL Basic Kit. | Exposure time: 10s.

Phospho-PAK4/PAK5/PAK6-S474/S560/S602 Rabbit Recombinant Antibody (84-605)

CATALOG NUMBER: 84-605

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
NIH/3T3 treated with PMA,C6 treated with PMA
Predicted Molecular Weight:
64, 80, 75 kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PAK4/PAK5/PAK6
Additional Names:
PAK4; KIAA1142; PAK5KIAA1264; PAK6; Phospho-PAK4/PAK5/PAK6-S474/S560/S602
Protein Accession Number:
O96013(PAK4-Antigen),Q9P286(PAK5),Q9NQU5(PAK6)
Ncbi Gene Id Number:
10298/57144/56924
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-605

List Size:
100 uL

List Price:

$540.00

Datasheet
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