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Western blot analysis of lysates from C2C12 cells using Phospho-PI3 Kinase p55-Y199 Rabbit mAb at 1:5000 dilution. C2C12 cells were treated with Hydrogen Peroxide (2 nM) at 37℃ for 15 minutes after serum-starvation overnight. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 60s.
Confocal imaging of C2C12 cells (treated with H2O2) and C2C12 (untreated) cells using Phospho-PI3 Kinase p55-Y199 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
Western blot analysis of lysates from C2C12 cells using Phospho-PI3 Kinase p55-Y199 Rabbit mAb at 1:5000 dilution. C2C12 cells were treated with Hydrogen Peroxide (2 nM) at 37℃ for 15 minutes after serum-starvation overnight. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 60s.

Phospho-PI3 Kinase p55-Y199 Rabbit Recombinant Antibody (84-726)

CATALOG NUMBER: 84-726

Clonality:
Monoclonal
Tested Applications:
ELISA, IF/ICC, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF/ICC, WB
Application Note:
WB,1:2000 - 1:6000, IF/ICC,1:50 - 1:200, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
55kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS with 0.09% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PIK3R3
Additional Names:
PIK3R3; Phospho-PI3KP55γ-Y199
Protein Accession Number:
Q92569
Ncbi Gene Id Number:
8503
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-726

List Size:
100 uL

List Price:

$540.00

Datasheet
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