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Western blot analysis of lysates from A-431 cells, using Phospho-Rac1/Cdc42-S71 Rabbit mAb at 1:1000 dilution. A-431 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% BSA. | Detection: ECL Enhanced Kit. | Exposure time: 3min.
Western blot analysis of lysates from A-431 cells, using Phospho-Rac1/Cdc42-S71 Rabbit mAb at 1:1000 dilution. A-431 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% BSA. | Detection: ECL Enhanced Kit. | Exposure time: 3min.

Phospho-Rac1/Cdc42-S71 Rabbit Recombinant Antibody (84-587)

CATALOG NUMBER: 84-587

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:500 - 1:2000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
A-431
Predicted Molecular Weight:
24kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CDC42/RAC1
Additional Names:
CDC42Hs; G25K; TKS; Phospho-Rac1/Cdc42-S71
Protein Accession Number:
P60953/P63000
Ncbi Gene Id Number:
998/5879
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-587

List Size:
100 uL

List Price:

$540.00

Datasheet
Shipping Info

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