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Western blot analysis of lysates from L-929,using Phospho-RIP3-T231/S232 Rabbit mAb at 1:700 dilution. L-929 cells were treated with 20 ng/ml mouse TNF-a,100 nM BV-6 and 20 μM z-VAD for 5 or 6 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Enhanced Kit. | Exposure time: 60s.
Western blot analysis of lysates from L-929,using Phospho-RIP3-T231/S232 Rabbit mAb at 1:700 dilution. L-929 cells were treated with 20 ng/ml mouse TNF-a,100 nM BV-6 and 20 μM z-VAD for 5 or 6 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Enhanced Kit. | Exposure time: 60s.

Phospho-RIP3-T231/S232 Rabbit Recombinant Antibody (84-691)

CATALOG NUMBER: 84-691

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
L-929 treated with mouse TNF-α,BV-6 and z-VAD
Predicted Molecular Weight:
53kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
Ripk3
Additional Names:
Rip3; 2610528K09Rik; Phospho-RIP3-T231/S232
Protein Accession Number:
Q9QZL0
Ncbi Gene Id Number:
56532
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Enables NF-kappaB-inducing kinase activity and protein serine/threonine kinase activity. Involved in several processes, including execution phase of necroptosis; hematopoietic or lymphoid organ development; and regulation of programmed cell death. Acts upstream of or within several processes, including I-kappaB kinase/NF-kappaB signaling; positive regulation of intrinsic apoptotic signaling pathway; and positive regulation of protein deacetylation. Located in cytoplasm and nucleus. Is expressed in several structures, including alimentary system; hemolymphoid system gland; male reproductive gland or organ; primitive streak; and turbinate bone primordium. Orthologous to human RIPK3 (receptor interacting serine/threonine kinase 3).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-691

List Size:
100 uL

List Price:

$540.00

Datasheet
Shipping Info

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