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Western blot analysis of lysates from HT-29 cells, using Phospho-RIPK1-S166 Rabbit mAb at 1:4000 dilution. HT-29 cells were treated with Z-VAD (50uM), TNF-α (50ng/ml) and BV-6 (200nM) at 37℃ for 5 hour. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30s.
Western blot analysis of lysates from HT-29 cells, using Phospho-RIPK1-S166 Rabbit mAb at 1:4000 dilution. HT-29 cells were treated with Z-VAD (50uM), TNF-α (50ng/ml) and BV-6 (200nM) at 37℃ for 5 hour. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30s.

Phospho-RIPK1-S166 Rabbit Recombinant Antibody (84-719)

CATALOG NUMBER: 84-719

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:2000 - 1:5000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
HT-29 treated by Z-VAD,TNF-α and BV-6
Predicted Molecular Weight:
75kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
RIPK1
Additional Names:
RIP; RIP1; AIEFL; IMD57; RIP-1; Phospho-RIPK1-S166
Protein Accession Number:
Q13546
Ncbi Gene Id Number:
8737
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein plays a role in inflammation and cell death in response to tissue damage, pathogen recognition, and as part of developmental regulation. RIPK1/RIPK3 kinase-mediated necrosis is referred to as necroptosis. Genetic disruption of this gene in mice results in death shortly after birth.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-719

List Size:
100 uL

List Price:

$540.00

Datasheet
Shipping Info

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