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Western blot analysis of various lysates using Phospho-SMAD1-S463/S465+SMAD5-S463/S465+SMAD9-S465/S467 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. HeLa cells and NIH/3T3 cells were treated with BMP4 (50 ng/mL) at 37℃ for 30 minutes. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30s.
Western blot analysis of lysates from C6 cells using Phospho-SMAD1-S463/S465+SMAD5-S463/S465+SMAD9-S465/S467 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. C6 cells were treated with TGF beta 3 (10 ng/mL) and MG132(2 μM) at 37℃ for 20 minutes. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3 % nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Phospho-SMAD1-S463/S465+SMAD5-S463/S465+SMAD9-S465/S467 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Phospho-SMAD1-S463/S465+SMAD5-S463/S465+SMAD9-S465/S467 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Phospho-SMAD1-S463/S465+SMAD5-S463/S465+SMAD9-S465/S467 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Moue colon tissue using Phospho-SMAD1-S463/S465+SMAD5-S463/S465+SMAD9-S465/S467 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Moue kidney tissue using Phospho-SMAD1-S463/S465+SMAD5-S463/S465+SMAD9-S465/S467 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Phospho-SMAD1-S463/S465+SMAD5-S463/S465+SMAD9-S465/S467 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using Phospho-SMAD1-S463/S465+SMAD5-S463/S465+SMAD9-S465/S467 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using Phospho-SMAD1-S463/S465+SMAD5-S463/S465+SMAD9-S465/S467 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Confocal imaging of HeLa cells (treated with BMP2) and HeLa cells (untreated) using Phospho-SMAD1-S463/S465+SMAD5-S463/S465+SMAD9-S465/S467 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). The cells were counterstained with α-tubulin Mouse mAb followed by incubation with PSFlo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Western blot analysis of various lysates using Phospho-SMAD1-S463/S465+SMAD5-S463/S465+SMAD9-S465/S467 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. HeLa cells and NIH/3T3 cells were treated with BMP4 (50 ng/mL) at 37℃ for 30 minutes. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30s.

Phospho-SMAD1-S463/S465+SMAD5-S463/S465+SMAD9-S465/S467 Rabbit Recombinant Antibody (84-754)

CATALOG NUMBER: 84-754

Clonality:
Monoclonal
Tested Applications:
ELISA, IF/ICC, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic phosphorylated peptide around S463/S465//S467 of human SMAD1/SMAD5/SMAD9.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF/ICC, IHC-P, WB
Application Note:
WB,1:1000 - 1:2000, IHC-P,1:200 - 1:800, IF/ICC,1:100 - 1:400, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
52kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SMAD1/SMAD5/SMAD9
Additional Names:
BSP1; MADH1; MADR1; JV4-1; DWFC; JV5-1; MADH5; MADH6; MADH9; SMAD8; Phospho-SMAD1-S463/S465+SMAD5-S463/S465+SMAD9-S465/S467
Protein Accession Number:
Q15797/Q99717/O15198
Ncbi Gene Id Number:
4086/4090/4093
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-754

List Size:
100 uL

List Price:

$540.00

Datasheet
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