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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Smad5-S465 Rabbit mAb at 1:2000 dilution. NIH/3T3 cells were treated with BMP4(50ng/ml) at 37℃ for 6 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 180s.
Western blot analysis of lysates from C6 cells using Phospho-Smad5-S465 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. C6 cells were treated with TGFβ3 (10 ng/mL) and MG132 (10 μM) for 30 minutes after serum starvation. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3 % nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 20 s.
Western blot analysis of lysates from HT-1080 cells using Phospho-Smad5-S465 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. HT-1080 cells were treated with BMP2 (50 ng/mL) for 30 minutes after serum starvation. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3 % nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 90 s.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Smad5-S465 Rabbit mAb at 1:2000 dilution. NIH/3T3 cells were treated with BMP4(50ng/ml) at 37℃ for 6 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 180s.

Phospho-Smad5-S465 Rabbit Recombinant Antibody (84-693)

CATALOG NUMBER: 84-693

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:1000 - 1:5000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
NIH/3T3 treated with BMP4,C6 treated with TGFβ and MG132
Predicted Molecular Weight:
52kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SMAD5
Additional Names:
DWFC; JV5-1; MADH5; Phospho-Smad5-S465
Protein Accession Number:
Q99717
Ncbi Gene Id Number:
4090
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-693

List Size:
100 uL

List Price:

$540.00

Datasheet
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