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Western blot analysis of lysates from TF-1 cells using Phospho-STAT5-Y694 Rabbit mAb at 1:12000 dilution incubated at room temperature for 1.5 hours. TF-1 cells were treated with hGM-CSF (100 ng/mL) at 37℃ for 10 minutes after serum-starvation overnight. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3 % nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 10s.
Western blot analysis of lysates from TF-1 cells using Phospho-STAT5-Y694 Rabbit mAb at 1:12000 dilution incubated at room temperature for 1.5 hours. TF-1 cells were treated with hGM-CSF (100 ng/mL) at 37℃ for 10 minutes after serum-starvation overnight. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3 % nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 10s.

Phospho-STAT5-Y694 Rabbit Recombinant Antibody (84-770)

CATALOG NUMBER: 84-770

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:12000 - 1:72000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
TF-1 cells were treated with hGM-CSF
Predicted Molecular Weight:
90kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
STAT5A/STAT5B
Additional Names:
MGF; STAT5; stat5a/stat5b; Phospho-STAT5-Y694
Protein Accession Number:
P42229/P51692
Ncbi Gene Id Number:
6776/6777
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Carries out a dual signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-770

List Size:
100 uL

List Price:

$540.00

Datasheet
Shipping Info

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