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IHC staining of breast carcinoma with phosphotyrosine antibody (PY20). Note cell surface and cytoplasmic staining.
SDS-PAGE Analysis of Purified, BSA-Free Phosphotyrosine Antibody (clone PY20). Confirmation of Integrity and Purity of the Antibody.
IHC staining of breast carcinoma with phosphotyrosine antibody (PY20). Note cell surface and cytoplasmic staining.

Phosphotyrosine Antibody [PY20]

CATALOG NUMBER: 33-259

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC, IP, WB
Host Species:
Mouse
Species Reactivity:
All Species
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
All Species
Immunogen:
Phosphotyrosine conjugated to KLH was used as the immunogen for this antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC, IP, WB
Application Note:
Flow Cytometry: 0.5-1 ug/million cells
IF: 1-2 ug/ml
WB: 0.5-1 ug/ml
IP: 1-2ug/500ug protein lysate
IHC (FFPE): 1-2 ug/ml for 30 minutes at RT (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Phosphotyrosine antibody to be titered up or down for optimal performance.

1. No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2b
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Gene Id Number:
NotApplicable
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This antibody shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-259

List Size:
100 ug

List Price:

$626.00

Datasheet
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