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Western blot analysis of PHOX2A in rat brain tissue lysate with PHOX2A antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of PHOX2A in human brain tissue with PHOX2A antibody at 2.5 μg/mL.
Immunofluorescence of PHOX2A in human brain tissue with PHOX2A antibody at 20 μg/mL.
Western blot analysis of PHOX2A in rat brain tissue lysate with PHOX2A antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

PHOX2A Antibody

CATALOG NUMBER: 6827

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PHOX2A antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human PHOX2A.
The immunogen is located within the last 50 amino acids of PHOX2A.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
PHOX2A antibody can be used for detection of PHOX2A by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
PHOX2A antibody is predicted to not cross-react with other paired homeobox family members.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
PHOX2A Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PHOX2A Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PHOX2A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PHOX2A
Additional Names:
PHOX2A Antibody: ARIX, FEOM2, NCAM2, PMX2A, CFEOM2, ARIX, Paired mesoderm homeobox protein 2A, ARIX1 homeodomain protein
Protein Accession Number:
NP_005160
Protein Gi Number:
46249382
Ncbi Gene Id Number:
401
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PHOX2A Antibody: PHOX2A and PHOX2B (Paired mesoderm homeobox protein) are closely related, paired-homeodomain transcription factors that function as determinants of the noradrenergic phenotype during embryogenesis. PHOX2 proteins are crucial for the regulation of endogenous hydroxylases in neural crest cells and promote sympathetic neuron generation. Human PHOX2B contains one DNA binding homeobox domain and is required for the differentiation of all central and nonperipheral noradrenergic centers in the brain. In contrast, PHOX2A controls only the differentiation of the main noradrenergic center of the brain. Regulation of PHOX2 may have therapeutic utility in aging or disorders involving degeneration of noradrenergic neurons.
Background Reference 1:
Stanke M, Junghans D, Geissen M, et al. The Phox2 homeodomain proteins are sufficient to promote the development of sympathetic neurons. Development 1999; 126:4087-94.
Background Reference 2:
Young HM, Ciampoli D, Hsuan J, et al. Expression of Ret-, p75(NTR)-, Phox2a-, Phox2b-, and tyrosine hydroxalase-immunoreactivity by undifferentiated neural crest-derived cells and different classes of enteric neurons in the embryonic mouse gut. Dev. Dyn. 1999; 216:137-52.
Background Reference 3:
Pattyn A, Morin X, Cremer H, et al. The homeobox gene Phox2b is essential for the development of autonomic neural crest derivatives. Nature 1999; 399:366-70.
Background Reference 4:
Coppola E, d'Autréaux F, Rijli FM, et al. Ongoing roles of Phox2 homeodomain transcription factors during neuronal differentiation. Development 2010; 137:4211-20.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6827

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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