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Western blot analysis of PHPT1 using rabbit polyclonal PHPT1 Antibody (Human C-term) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHPT1 gene (Lane 2).
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PHPT1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of PHPT1 using rabbit polyclonal PHPT1 Antibody (Human C-term) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHPT1 gene (Lane 2).

PHPT1 Antibody

CATALOG NUMBER: 61-451

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PHPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-117 amino acids from the C-terminal region of human PHPT1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
14 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PHPT1
Additional Names:
14 kDa phosphohistidine phosphatase, 313-, Phosphohistidine phosphatase 1, Protein janus-A homolog, PHPT1, PHP14
Protein Accession Number:
Q9NRX4
Protein Gi Number:
25008934
Ncbi Gene Id Number:
29085
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PHPT1 is an EDTA-insensitive phosphohistidine phosphatase that catalyzes the dephosphorylation of phosphopeptide I (Ek etal., 2002 [PubMed 12383260]).[supplied by OMIM].
Background Reference 1:
Lim,J., Cell 125 (4), 801-814 (2006)
Background Reference 2:
Mehrle,A., Nucleic Acids Res. 34 (DATABASE ISSUE), D415-D418 (2006)
Background Reference 3:
Ma,R.,Biochem. Biophys. Res. Commun. 337 (3), 887-891 (2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-451

List Size:
400 ul

List Price:

$495.00

Datasheet
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