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46-974 (1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-974 (1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PHR1 Antibody

CATALOG NUMBER: 46-974

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Rat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Cow
Immunogen:
The immunogen for this antibody is: PPDSALESPFEE-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 25kDa band observed in Rat Brain lysates (calculated MW of 25.2kDa according to Rat NP_742030.1 (Evectin-1) and of 27.2kDa according to Human NP_067023.1). Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is expected to recognize reported isoforms a (NP_067023.1) and c (NP_001123505.1).
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PLEKHB1
Additional Names:
PLEKHB1, pleckstrin homology domain containing, family B (evectins) member 1, KPL1, PHR1, PHRET1, PH domain containing protein in retina 1, PH domain containing, retinal 1, evectin-1
Protein Accession Number:
NP_067023.1,NP_001123506.1,NP_001123505.1,NP_001123507.1,NP_001123508.1,
Protein Gi Number:
10864013
Ncbi Gene Id Number:
58473
Background Reference 1:
Andrews KL, Potdar PD, Nettesheim P, Ostrowski LE. KPL1, which encodes a novel PH domain-containing protein, is induced during ciliated cell differentiation of rat tracheal epithelial cells. Exp Lung Res. 2000 Jun;26(4):257-71.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-974

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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