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Western blot analysis of PHTNS Antibody in Ramos cell line lysates (35ug/lane)
PHTNS Antibody IHC analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of PHTNS Antibody in Ramos cell line lysates (35ug/lane)

PHTNS Antibody

CATALOG NUMBER: 64-226

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PHTNS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-220 amino acids from the N-terminal region of human PHTNS.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
68 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PPP1R18
Additional Names:
Phostensin, Protein phosphatase 1 F-actin cytoskeleton-targeting subunit, Protein phosphatase 1 regulatory subunit 18, PPP1R18, HKMT1098, KIAA1949
Protein Accession Number:
Q6NYC8
Protein Gi Number:
68052323
Ncbi Gene Id Number:
170954
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PHTNS interacts with regulatory subunits that target the enzyme to different cellular locations and change its activity toward specific substrates. Phostensin is a regulatory subunit that targets PP1 to F-actin cytoskeleton
Background Reference 1:
Lai, N.S., et al. Biochem. Biophys. Res. Commun. 387(4):676-681(2009)
Background Reference 2:
Barcellos, L.F., et al. PLoS Genet.(10), E1000696 (2009)
Background Reference 3:
Kao, S.C., et al. Biochem. Biophys. Res. Commun. 356(3):594-598(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-226

List Size:
400 ul

List Price:

$495.00

Datasheet
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