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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in K562 cell line lysates (35ug/lane).
PI15 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.

PI15 Antibody

CATALOG NUMBER: 56-994

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PI15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-63 amino acids from the N-terminal region of human PI15.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
29 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PI15
Additional Names:
Peptidase inhibitor 15, PI-15, 25 kDa trypsin inhibitor, p25TI, Cysteine-rich secretory protein 8, CRISP-8, SugarCrisp, PI15, CRISP8, P25TI
Protein Accession Number:
O43692
Ncbi Gene Id Number:
51050
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a trypsin inhibitor. The protein sharessimilarity to insect venom allergens, mammalian testis-specificproteins and plant pathogenesis-related proteins. It is frequentlyexpressed in human neuroblastoma and glioblastoma cell lines, andthus may play a role in the central nervous system. [provided byRefSeq].
Background Reference 1:
Melzer, D., et al. PLoS Genet.(5), E1000072 (2008) :
Background Reference 2:
Kaplan, F., et al. Am. J. Physiol. 276 (6 PT 1), L1027-L1036 (1999) :
Background Reference 3:
Yamakawa, T., et al. Biochim. Biophys. Acta 1395(2):202-208(1998)
Background Reference 4:
Yamakawa, T., et al. Biochim. Biophys. Acta 1395(2):202-208(1998)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-994

List Size:
400 ul

List Price:

$495.00

Datasheet
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