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Fluorescent image of Hela cell stained with PI3KCD Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PI3KCD primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).PI3KCD immunoreactivity is localized to Cytoplasm significantly.
Western blot analysis of anti-PI3KCD Antibody Pab (AP8020d) in 293 cell line lysates.
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PI3KCD Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Fluorescent image of Hela cell stained with PI3KCD Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PI3KCD primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).PI3KCD immunoreactivity is localized to Cytoplasm significantly.

PI3KCD Antibody

CATALOG NUMBER: 63-347

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This PI3KCD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85-114 amino acids from the N-terminal region of human PI3KCD.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For IF starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
119 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PIK3CD
Additional Names:
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform, PI3-kinase subunit delta, PI3K-delta, PI3Kdelta, PtdIns-3-kinase subunit delta, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta, PtdIns-3-kinase subunit p110-delta, p110delta, PIK3CD
Protein Accession Number:
O00329
Ncbi Gene Id Number:
5293
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. See MIM 602838. The class I PI3Ks display a broad phosphoinositide lipid substrate specificity and include p110-alpha , p110-beta, and p110-gamma. p110-alpha and p110-beta interact with SH2/SH3-domain-containing p85 adaptor proteins and with GTP-bound Ras.
Background Reference 1:
Khan, N.A., et al., J. Neurovirol. 9(6):584-593 (2003).
Background Reference 2:
Sadhu, C., et al., J. Immunol. 170(5):2647-2654 (2003).
Background Reference 3:
Deregibus, M.C., et al., J. Biol. Chem. 277(28):25195-25202 (2002).
Background Reference 4:
Cook, J.A., et al., J. Immunol. 169(1):254-260 (2002).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-347

List Size:
400 ul

List Price:

$495.00

Datasheet
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