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Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in CEM cell line lysates (35ug/lane).
Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.

PI4K2A Antibody

CATALOG NUMBER: 58-816

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Zebrafish, Rat
Immunogen:
This PI4K2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 387-415 amino acids from the C-terminal region of human PI4K2A.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
54 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PI4K2A
Additional Names:
Phosphatidylinositol 4-kinase type 2-alpha, Phosphatidylinositol 4-kinase type II-alpha, PI4K2A
Protein Accession Number:
Q9BTU6
Protein Gi Number:
74752344
Ncbi Gene Id Number:
55361
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Phosphatidylinositolpolyphosphates (PtdInsPs) arecentrally involved in many biologic processes, ranging from cellgrowth and organization of the actin cytoskeleton to endo- andexocytosis. PI4KII phosphorylates PtdIns at the D-4 position, anessential step in the biosynthesis of PtdInsPs (Barylko et al.,2001 [PubMed 11244087]).
Background Reference 1:
Li, J., et al. Oncogene 29(17):2550-2559(2010)
Background Reference 2:
Qin, Y., et al. J. Biol. Chem. 284(34):22544-22548(2009)
Background Reference 3:
Salazar, G., et al. J. Biol. Chem. 284(3):1790-1802(2009)
Background Reference 4:
Craige, B., et al. Mol. Biol. Cell 19(4):1415-1426(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-816

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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