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Western blot analysis of PIAS4 in HL60 cell lysate with PIAS4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of PIAS4 in rat brain tissue with PIAS4 antibody at 5 μg/mL.
Western blot analysis of PIAS4 in HL60 cell lysate with PIAS4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

PIAS4 Antibody

CATALOG NUMBER: 5747

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PIAS4 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human PIAS4.
The immunogen is located within amino acids 50 - 100 of PIAS4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
PIAS4 antibody can be used for detection of PIAS4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1209 - HL60 Cell Lysate
Predicted Molecular Weight:
Predicted: 56 kDa
Observed: 56 kDa
Purification:
PIAS4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PIAS4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PIAS4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PIAS4
Additional Names:
PIAS4 Antibody: PIASY, Piasg, ZMIZ6, PIASG, E3 SUMO-protein ligase PIAS4, PIASy, PIAS-gamma
Protein Accession Number:
NP_056981
Protein Gi Number:
24850133
Ncbi Gene Id Number:
51588
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PIAS4 Antibody: The PIAS (protein inhibitor of activated STAT) proteins play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS4. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS4 interacts with p53 and blocks its ability to induce Bax and p21 transcription. PIAS4 is also important in the control of the ubiquitin-dependent proteasomal degradation of the Ets-1 transcription factor. PIAS4 has been implicated in the DNA-damage response pathway and is thought to work in combination with PIAS1 for the productive association of 53BP1, BRCA1 and RNF168.
Background Reference 1:
Shuai K and Liu B. Regulation of gene-activation pathways by PIAS proteins in the immune system. Nat. Rev. Immunol. 2005; 5:593-605.
Background Reference 2:
Nelson V, Davis GE, and Maxwell SA. A putative protein inhibitor of activated STAT (PIASy) interacts with p53 and inhibits p53-,ediated transactivation but not apoptosis. Apoptosis 2001; 6:221-34.
Background Reference 3:
Nishida T, Terashima M, Fukami K, et al. PIASy controls ubiquitin-dependent proteasomal degradation of Ets-1. Biochem. J. 2007; 405:481-8.
Background Reference 4:
Galanty Y, Belotserkovskaya R, Coates J, et al. Mammalian SUMO E3-ligases PIAS1 and PIAS4 promote responses to DNA double-strand breaks. Nature 2009; 462:935-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5747

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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