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45-110 (1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-110 (5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-110 (5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-110 (1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

PICK1 Antibody

CATALOG NUMBER: 45-110

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-HYNKLQADPKQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 50kDa band observed in Mouse Brain lysates (calculated MW of 46.6kDa according to NP_036539.1, NP_001034672.1and NP_001034673.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Colon and Prostate. Recommended concentration: 5ug/ml.
Specificity:
Reported variants represent identical protein (NP_036539.1; NP_001034672.1; NP_001034673.1).
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PICK1
Additional Names:
PICK1, protein interacting with PRKCA 1, MGC15204, PRKCABP , OTTHUMP00000028509, PRKCA-binding protein, protein interacting with C kinase 1, protein kinase C, alpha binding protein
Protein Accession Number:
NP_036539.1
Protein Gi Number:
7110697
Ncbi Gene Id Number:
9463
Background Reference 1:
Steinberg JP, Takamiya K, Shen Y, Xia J, Rubio ME, Yu S, Jin W, Thomas GM, Linden DJ, Huganir RL. Targeted in vivo mutations of the AMPA receptor subunit GluR2 and its interacting protein PICK1 eliminate cerebellar long-term depression. Neuron. 2006 Mar 16;49(6):845-60.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-110

List Size:
0.1 mg

List Price:

$495.00

Datasheet
Shipping Info

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