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Western blot analysis of PID expression in HeLa whole cell lysates in the absence (A) or presence (B) of blocking peptide with PID antibody at 1 μg/mL.
Immunocytochemistry staining of HeLa using PID antibody at 10 μg/mL.
Immunofluorescence of PID in HeLa cells with PID antibody at 10 ug/mL.
Immunohistochemistry of PID in human small intestine tissue with PID antibody at 2.5 μg/ml.
Western blot analysis of PID expression in HeLa whole cell lysates in the absence (A) or presence (B) of blocking peptide with PID antibody at 1 μg/mL.
Green Product
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PID Antibody

CATALOG NUMBER: 2443

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PID antibody was raised against a 17 amino acid peptide near the carboxy terminus of human PID.
The immunogen is located within the last 50 amino acids of PID.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Application Note:
PID antibody can be used for detection of PID by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and Immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
PID antibody is predicted to not cross-react with MTA2
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Predicted Molecular Weight:
Predicted: 73 kDa
Observed: 75 kDa
Purification:
PID Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PID Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PID antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MTA2
Additional Names:
PID Antibody: PID, MTA1L1, PID, Metastasis-associated protein MTA2, Metastasis-associated 1-like 1, MTA1-L1 protein
Protein Accession Number:
AAG02241
Protein Gi Number:
9931638
Ncbi Gene Id Number:
9219
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PID Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 pathway, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The transcriptional activity of p53 is modulated by protein stability and acetylation. PID/MTA2, also termed MTA1-L1, was found to be a subunit of nucleosome remodeling and deacetylating (NRD/NuRD) complex. PID/MTA2 modulates the enzymatic activity of the histone deacetylase complex and its expression reduces the levels of acetylated p53. Deacetylation of p53 by PID/MTA2 represses p53-dependent transcriptional activation and modulates p53-mediated cell growth arrest and apoptosis. PID/MTA2 is ubiquitously expressed in human tissues.
Background Reference 1:
Matsuoka S, Huang M, and Elledge SJ. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science 1998; 282:1893-7.
Background Reference 2:
Tanaka H, Arakawa H, Yamaguchi T, et al. A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage. Nature 2000; 404:42-9.
Background Reference 3:
Oda E, Ohki R, Murasawa H, et al. Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis. Science 2000; 288:1053-8.
Background Reference 4:
Oda K, Arakawa H, Tanaka T, et al. p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53. Cell 2000;102:849-62.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2443

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

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