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Western blot analysis of PIERCE1 in A20 cell lysate with PIERCE1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of PIERCE1 in A20 cells with PIERCE1 antibody at 2.5 μg/mL.
Western blot analysis of PIERCE1 in A20 cell lysate with PIERCE1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

PIERCE1 Antibody

CATALOG NUMBER: 6721

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
PIERCE1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human PIERCE1.
The immunogen is located within the first 50 amino acids of PIERCE1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
PIERCE1 antibody can be used for detection of PIERCE1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested.
Specificity:
PIERCE1 antibody is predicted to not cross-react with other UPF0691 family members.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Positive Control 2:
Cat. No. 17-208 - A-20 Cell Slide
Purification:
PIERCE1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PIERCE1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PIERCE1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
C9orf116
Additional Names:
PIERCE1 Antibody: PIERCE1, RbEST47, UPF0691 protein C9orf116, p53-induced expression in RB-null cells protein 1, Pierce1
Protein Accession Number:
Q5BN46
Protein Gi Number:
115430217
Ncbi Gene Id Number:
138162
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PIERCE1 Antibody: PIERCE1, also known as C9orf116, is an important p53 target gene contributing to normal DNA damage response and may play crucial roles in maintaining genomic integrity against genotoxic stresses, including UVC irradiation. PIERCE1 exists as two alternatively spliced isoforms and is predominantly expressed in human small cell lung cancer. PIERCE1 knockdown induces down-regulation of proapoptotic genes including Ei24, Apaf1 and PTEN, suggesting that PIERCE1 is a proapoptotic gene. It is helpful for in-depth understanding of p53 pathways and the design of new cancer therapies.
Background Reference 1:
Sung YH, Kim HJ, Devkota S, et al. Pierce1, a novel p53 target gene contributing to the ultraviolet-induced DNA damage response. Cancer Res. 2010; 70:10454-63.
Background Reference 2:
Zhou BB and Elledge SJ. The DNA damage response: putting checkpoints in perspective. Nature 2000; 408:433-9.
Background Reference 3:
Sung YH, Kim HJ, Lee HW, et al. Identification of a novel Rb-regulated gene associated with the cell cycle. Mol. Cells 2007; 24:409-15.
Background Reference 4:
Kakiuchi S, Daigo Y, Tsunoda T, et al. 2003. Genome-wide analysis of organ-preferential metastasis of human small cell lung cancer in mice. Mol. Cancer Res. 1:485-99.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6721

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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