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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
PIGA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).

PIGA Antibody

CATALOG NUMBER: 56-383

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PIGA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 455-484 amino acids from the C-terminal region of human PIGA.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
54 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PIGA
Additional Names:
Phosphatidylinositol N-acetylglucosaminyltransferase subunit A, GlcNAc-PI synthesis protein, Phosphatidylinositol-glycan biosynthesis class A protein, PIG-A, PIGA
Protein Accession Number:
P37287
Protein Gi Number:
585696
Ncbi Gene Id Number:
5277
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein required for synthesis ofN-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the firstintermediate in the biosynthetic pathway of GPI anchor. The GPIanchor is a glycolipid found on many blood cells and which servesto anchor proteins to the cell surface. Paroxysmal nocturnalhemoglobinuria, an acquired hematologic disorder, has been shown toresult from mutations in this gene. Alternate splice variants havebeen characterized. A related pseudogene is located on chromosome12.
Background Reference 1:
Borowitz, M.J., et al. CytometryClin Cytom 78(4):211-230(2010)
Background Reference 2:
Peruzzi, B., et al. Mutat. Res. 705(1):3-10(2010)
Background Reference 3:
Araten, D.J., et al. Mutat. Res. 686 (1-2), 1-8 (2010) :
Background Reference 4:
Iida, Y., et al. Blood 83(11):3126-3131(1994)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-383

List Size:
400 ul

List Price:

$495.00

Datasheet
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