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Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human hepatocarcinoma with PIGR Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
PIGR Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of PIGR Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of HepG2 cells using PIGR Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

PIGR Antibody

CATALOG NUMBER: 63-514

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PIGR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 646-672 amino acids from the C-terminal region of human PIGR.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
83 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PIGR
Additional Names:
Polymeric immunoglobulin receptor, PIgR, Poly-Ig receptor, Hepatocellular carcinoma-associated protein TB6, Secretory component, PIGR
Protein Accession Number:
P01833
Protein Gi Number:
150421625
Ncbi Gene Id Number:
5284
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PIGR binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.
Background Reference 1:
Ewing,R.M., et.al., Mol. Syst. Biol. 3, 89 (2007)
Background Reference 2:
Orzech,E., Cohen,S., et.al., J. Biol. Chem. 275 (20), 15207-15219 (2000)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-514

List Size:
400 ul

List Price:

$495.00

Datasheet
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