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46-185 (2ug/ml) staining of paraffin embedded Human Placenta. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
46-185 (5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-185 (2ug/ml) staining of paraffin embedded Human Placenta. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

PIK3C2A Antibody

CATALOG NUMBER: 46-185

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog
Immunogen:
The immunogen for this antibody is: C-TVKWYQLTAATYL
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave no signal but low background in Human Testis and Molt-4 lysates at up to 1ug/ml.Immunohistochemistry:In paraffin embedded Human Placenta shows cytoplasm staining of cytotrophoblasts and mesenchymal cells. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Heart. Recommended concentration: 5ug/ml.
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PIK3C2A
Additional Names:
PIK3C2A, CPK, PI3-K-C2A, PI3K-C2alpha, PI3-K-C2(ALPHA), phosphoinositide-3-kinase, class 2, alpha polypeptide, C2-containing phosphatidylinositol kinase, DKFZp686L193, MGC142218,
Protein Accession Number:
NP_002636.2
Protein Gi Number:
157671929
Ncbi Gene Id Number:
5286
Background Reference 1:
Molz L, Chen YW, Hirano M, Williams LT. Cpk is a novel class of Drosophila PtdIns 3-kinase containing a C2 domain. J Biol Chem. 1996 Jun 7;271(23):13892-9.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-185

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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