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Western blot analysis of PIK3R1 using rabbit polyclonal PIK3R1 Antibody (Y580) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIK3R1 gene (Lane 2).
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PIK3R1-pY580, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of PIK3R1 using rabbit polyclonal PIK3R1 Antibody (Y580) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIK3R1 gene (Lane 2).

PIK3R1 Antibody

CATALOG NUMBER: 63-349

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Bovine, Mouse, Rat
Immunogen:
This PIK3R1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 558-587 amino acids from human PIK3R1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
84 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PIK3R1
Additional Names:
Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha, PIK3R1, GRB1
Protein Accession Number:
P27986
Protein Gi Number:
118572681
Ncbi Gene Id Number:
5295
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance.
Background Reference 1:
Kobayashi, H., et al., J. Biol. Chem. 279(8):6371-6379 (2004).
Background Reference 2:
Liu, H., et al., J. Cell Biol. 164(4):603-612 (2004).
Background Reference 3:
Sun, M., et al., J. Biol. Chem. 278(44):42992-43000 (2003).
Background Reference 4:
Khan, N.A., et al., J. Neurovirol. 9(6):584-593 (2003).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-349

List Size:
400 ul

List Price:

$495.00

Datasheet
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