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Western blot analysis of PIK3R2 using rabbit polyclonal PIK3R2 Antibody (Y467) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIK3R2 gene (Lane 2).
Formalin-fixed and paraffin-embedded human brain tissue reacted with PIK3R2-pY467, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of PIK3R2 using rabbit polyclonal PIK3R2 Antibody (Y467) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIK3R2 gene (Lane 2).

PIK3R2 Antibody

CATALOG NUMBER: 63-353

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This PIK3R2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-474 amino acids from human PIK3R2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
82 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PIK3R2
Additional Names:
Phosphatidylinositol 3-kinase regulatory subunit beta, PI3-kinase regulatory subunit beta, PI3K regulatory subunit beta, PtdIns-3-kinase regulatory subunit beta, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta, PI3-kinase subunit p85-beta, PtdIns-3-kinase regulatory subunit p85-beta, PIK3R2
Protein Accession Number:
O00459
Ncbi Gene Id Number:
5296
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.PIK3R2 binds to activated Protein Tyrosine Kinases, which are phosphorylated, through its SH2 domain, and acts as an adaptor, mediating the association of the P110 catalytic unit to the plasma membrane.
Background Reference 1:
Khan, N.A., et al., J. Neurovirol. 9(6):584-593 (2003).
Background Reference 2:
Deregibus, M.C., et al., J. Biol. Chem. 277(28):25195-25202 (2002).
Background Reference 3:
Cook, J.A., et al., J. Immunol. 169(1):254-260 (2002).
Background Reference 4:
Park, I.W., et al., Blood 97(2):352-358 (2001).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-353

List Size:
400 ul

List Price:

$495.00

Datasheet
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